Composite

Part:BBa_J107251:Design

Designed by: Lorenzo Pasotti, Massimo Bellato, Angelica Frusteri   Group: Magni Lab   (2021-05-27)


HSL-inducible cassette for sgRNA (optimized) targeting R0040


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2087
    Illegal BsaI site found at 2265
    Illegal BsaI.rc site found at 706
    Illegal BsaI.rc site found at 1839


Design Notes

Registry parts.


Source

BioBrick Standard Assembly.

References